Sweta Sanguri Damodar Gupta


Mitochondrial dynamics is important for mitochondrial inheritance and for the maintenance of mitochondrial functions and thereby ensuring cell survival. Mitofusin 1 (Mfn1), Mitofusin 2 (Mfn2) and Optic Atrophy 1 (OPA1) are important proteins are required in fusion process of mitochondria. Whereas proteins like, Dynamin related protein 1 (Drp1) and mitochondrial fission 1 (Fis1) play important role in mitochondrial fission in cells. Ionizing radiation (IR) exposure to cells has been known to disrupt fission and fusion homeostasis directly resulting in mitochondrial fragmentation and reduction in respiratory chain functions. Therefore, protection of mitochondra from IR, is crucial in order to minimize damage to the cells. This damage to the cells may be minimized by restoration of mitochondrial structure and functions; and therefore the effect of radiomodifying agents that restores mitochondrial functioning is worth investigating. In the present study we have identified that mannan oligosaccharide (MOS) pretreatment to cells followed by IR exposure showed reduced alterations in mitochondrial dynamics with respect to irradiated cell. Moreover, pre-irradiation treatment of cells with MOS pretreatment to cells restored the levels of proteins responsible for both mitochondrial dynamics and ETC functions