The Green fluorescent protein chromophore housed inside the barrel has inspired scientists to use it as a marker to visualise cellular processes. Its emerging use in the synthetic form to develop dyes and understand photophysical and photochemical processes can also be seen. In this view, organic solvents are proving a better solvent system to study such processes. Though organic solvents are being used to study chromophore and its analogues, their influences on Green Fluorescent Protein are underestimated. Use of Green Fluorescent Protein in organic solvents can prove very beneficial from biological and industrial perspective. Its engineering can produce an organic solvent monitoring biosensor. In this review, organic solvent influences on GFP and its chromophore have been summarised and applications along with strategies to develop it as a biosensor have been proposed.